MDFI Protein (AA 1-251) (His tag)

Produktnummer ABIN7556699

Produktdetails

Target: MDFI (MyoD Family Inhibitor (MDFI))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-251

Spezies: Maus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses MDFI Protein ist gelabelt mit His tag.

Verwendungszweck: Custom-made recombinant Mdfi Protein expressed in mammalian cells.

Sequenz: MSQVSGQCPS RCDAPHGVPS AALDPAQTMS LLPGLEVARS THPVEASSEE GFPEEAAPSM PHDSGLRAQQ ALNSIDLDVP TEAVTCQPQG NPQGCTPLLP NGSSHDHLSE PGSAGHAGNG ALGGSKAHRK LQTHPSLGSQ AGRKSRGSAR SASQVPLQAQ EGKAPAVRIH RQTASPTCCL RNAQLSGTAL RSLRLESQGH RELNNKTLSQ SNNKKPGVAA HAAIIPALTR PKQNCHDPSL LPGTHGVGKE F Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Spezifität: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Produktmerkmale: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Reinheit: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Güteklasse: custom-made

Anwendungsinformationen

Applikationshinweise: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: 12 months

Antigendetails

Target: MDFI (MyoD Family Inhibitor (MDFI))

Andere Bezeichnung: Mdfi (MDFI Produkte)

Hintergrund: MyoD family inhibitor (Myogenic repressor I-mf),FUNCTION: Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis. Acts by associating with Myod family members and retaining them in the cytoplasm by masking their nuclear localization signals. Can also interfere with the DNA-binding activity of Myod family members. Plays an important role in trophoblast and chondrogenic differentiation. Regulates the transcriptional activity of TCF7L1/TCF3 by interacting directly with TCF7L1/TCF3 and preventing it from binding DNA. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways. {ECO:0000269|PubMed:11238923, ECO:0000269|PubMed:12192039, ECO:0000269|PubMed:8797820, ECO:0000269|PubMed:9799236}.

Molekulargewicht: 26.0 kDa

UniProt: P70331

AlphaFold: P70331

Pathways: Maintenance of Protein Location