KLHL25 Protein (AA 1-589) (His tag)

Produktnummer ABIN7554366

Recombinant KLHL25-Protein exprimiert in HEK-293 Cells.

Kurzübersicht für KLHL25 Protein (AA 1-589) (His tag) (ABIN7554366)

Target: KLHL25 (Kelch-Like 25 (KLHL25))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Güteklasse: custom-made

Produktdetails

Proteineigenschaft: AA 1-589

Aufreinigungstag / Konjugat: Dieses KLHL25 Protein ist gelabelt mit His tag.

Verwendungszweck: Predefined custom protein recombinant KLHL25 Protein expressed in mammalian cells.

Sequenz: MSVSVHETRK SRSSTGSMNV TLFHKASHPD CVLAHLNTLR KHCMFTDVTL WAGDRAFPCH RAVLAASSRY FEAMFSHGLR ESRDDTVNFQ DNLHPEVLEL LLDFAYSSRI AINEENAESL LEAGDMLQFH DVRDAAAEFL EKNLFPSNCL GMMLLSDAHQ CRRLYEFSWR MCLVHFETVR QSEDFNSLSK DTLLDLISSD ELETEDERVV FEAILQWVKH DLEPRKVHLP ELLRSVRLAL LPSDCLQEAV SSEALLMADE RTKLIMDEAL RCKTRILQND GVVTSPCARP RKAGHTLLIL GGQTFMCDKI YQVDHKAKEI IPKADLPSPR KEFSASAIGC KVYVTGGRGS ENGVSKDVWV YDTVHEEWSK AAPMLIARFG HGSAELENCL YVVGGHTSLA GVFPASPSVS LKQVEKYDPG ANKWMMVAPL RDGVSNAAVV SAKLKLFVFG GTSIHRDMVS KVQCYDPSEN RWTIKAECPQ PWRYTAAAVL GSQIFIMGGD TEFTAASAYR FDCETNQWTR IGDMTAKRMS CHALASGNKL YVVGGYFGTQ RCKTLDCYDP TSDTWNCITT VPYSLIPTAF VSTWKHLPA Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Spezifität: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Produktmerkmale: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Anwendungsinformationen

Applikationshinweise: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: 12 months

Antigendetails

Target: KLHL25 (Kelch-Like 25 (KLHL25))

Andere Bezeichnung: KLHL25

Hintergrund: Kelch-like protein 25 (Ectoderm-neural cortex protein 2) (ENC-2),FUNCTION: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex involved in various processes, such as translation homeostasis and lipid synthesis (PubMed:22578813, PubMed:27664236, PubMed:34491895). The BCR(KLHL25) ubiquitin ligase complex acts by mediating ubiquitination of hypophosphorylated EIF4EBP1 (4E-BP1): ubiquitination and subsequent degradation of hypophosphorylated EIF4EBP1 (4E-BP1) probably serves as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low (PubMed:22578813). The BCR(KLHL25) complex does not target EIF4EBP1 (4E-BP1) when it is hyperphosphorylated or associated with eIF4E (PubMed:22578813). The BCR(KLHL25) complex also acts as a regulator of lipid synthesis by mediating ubiquitination and degradation of ACLY, thereby inhibiting lipid synthesis (PubMed:27664236, PubMed:34491895). BCR(KLHL25)-mediated degradation of ACLY promotes fatty acid oxidation and is required for differentiation of inducible regulatory T (iTreg) cells (PubMed:34491895). {ECO:0000269|PubMed:22578813, ECO:0000269|PubMed:27664236, ECO:0000269|PubMed:34491895}.

Molekulargewicht: 65.9 kDa

UniProt: Q9H0H3

AlphaFold: Q9H0H3