custom-made
ASAP2
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
ASAP2
(ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 2 (ASAP2))
Protein-Typ
Recombinant
Proteineigenschaft
AA 1-1006
Spezies
Human
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses ASAP2 Protein ist gelabelt mit His tag.
Verwendungszweck
Made-to-order recombinant ASAP2 Protein expressed in mammalian cells.
Sequenz
MPDQISVSEF VAETHEDYKA PTASSFTTRT AQCRNTVAAI EEALDVDRMV LYKMKKSVKA INSSGLAHVE NEEQYTQALE KFGGNCVCRD DPDLGSAFLK FSVFTKELTA LFKNLIQNMN NIISFPLDSL LKGDLKGVKG DLKKPFDKAW KDYETKITKI EKEKKEHAKL HGMIRTEISG AEIAEEMEKE RRFFQLQMCE YLLKVNEIKI KKGVDLLQNL IKYFHAQCNF FQDGLKAVES LKPSIETLST DLHTIKQAQD EERRQLIQLR DILKSALQVE QKEDSQIRQS TAYSLHQPQG NKEHGTERNG SLYKKSDGIR KVWQKRKCSV KNGFLTISHG TANRPPAKLN LLTCQVKTNP EEKKCFDLIS HDRTYHFQAE DEQECQIWMS VLQNSKEEAL NNAFKGDDNT GENNIVQELT KEIISEVQRM TGNDVCCDCG APDPTWLSTN LGILTCIECS GIHRELGVHY SRMQSLTLDV LGTSELLLAK NIGNAGFNEI MECCLPAEDS VKPNPGSDMN ARKDYITAKY IERRYARKKH ADNAAKLHSL CEAVKTRDIF GLLQAYADGV DLTEKIPLAN GHEPDETALH LAVRSVDRTS LHIVDFLVQN SGNLDKQTGK GSTALHYCCL TDNAECLKLL LRGKASIEIA NESGETPLDI AKRLKHEHCE ELLTQALSGR FNSHVHVEYE WRLLHEDLDE SDDDMDEKLQ PSPNRREDRP ISFYQLGSNQ LQSNAVSLAR DAANLAKEKQ RAFMPSILQN ETYGALLSGS PPPAQPAAPS TTSAPPLPPR NVGKVQTASS ANTLWKTNSV SVDGGSRQRS SSDPPAVHPP LPPLRVTSTN PLTPTPPPPV AKTPSVMEAL SQPSKPAPPG ISQIRPPPLP PQPPSRLPQK KPAPGADKST PLTNKGQPRG PVDLSATEAL GPLSNAMVLQ PPAPMPRKSQ ATKLKPKRVK ALYNCVADNP DELTFSEGDV IIVDGEEDQE WWIGHIDGDP GRKGAFPVSF VHFIAD Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
Spezifität
If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
Produktmerkmale
Key Benefits:
Made to order protein - from design to production - by highly experienced protein experts.
Protein expressed in mammalian cells and purified in one-step affinity chromatography
The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Reinheit
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
custom-made
ASAP2
Spezies: Maus
Wirt: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
custom-made
ASAP2
Spezies: Human
Wirt: Cell-free protein synthesis (CFPS)
Recombinant
> 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, WB, SDS
custom-made
ASAP2
Spezies: Maus
Wirt: Cell-free protein synthesis (CFPS)
Recombinant
> 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, WB, SDS
Applikationshinweise
We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
The buffer composition is at the discretion of the manufacturer.
Handhabung
Avoid repeated freeze-thaw cycles.
Lagerung
-80 °C
Informationen zur Lagerung
Store at -80°C.
Haltbarkeit
12 months
Target
ASAP2
(ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 2 (ASAP2))
Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 (Development and differentiation-enhancing factor 2) (Paxillin-associated protein with ARF GAP activity 3) (PAG3) (Pyk2 C-terminus-associated protein) (PAP),FUNCTION: Activates the small GTPases ARF1, ARF5 and ARF6. Regulates the formation of post-Golgi vesicles and modulates constitutive secretion. Modulates phagocytosis mediated by Fc gamma receptor and ARF6. Modulates PXN recruitment to focal contacts and cell migration. {ECO:0000269|PubMed:10022920, ECO:0000269|PubMed:10749932, ECO:0000269|PubMed:11304556}.