S1PR1 Protein

Produktnummer ABIN7538533

Synthetic Nanodisc S1PR1-Protein exprimiert in Mammalian Cells.

Kurzübersicht für S1PR1 Protein (ABIN7538533)

Target: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Protein-Typ: Synthetic Nanodisc

Spezies: Human

Quelle: Mammalian Cells

Produktdetails

Verwendungszweck: Human S1PR1 full length protein-synthetic nanodisc

Produktmerkmale: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Anwendungsinformationen

Kommentare:

Advantages of Synthetic Nanodiscs:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations of Synthetic Nanodiscs:

• Intolerant to acids and high concentrations of divalent metal ions

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Haltbarkeit: 12 months

Antigendetails

Target: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Andere Bezeichnung: S1PR1

Hintergrund: The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Molekulargewicht: The human full length S1PR1 protein has a MW of 42.8kDa

UniProt: P21453

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2