CD63 Protein (CD63) (DYKDDDDK Tag)

Produktnummer ABIN7538173

MNP Membrane Nanoparticle CD63-Protein exprimiert in HEK-293 Cells.

Kurzübersicht für CD63 Protein (CD63) (DYKDDDDK Tag) (ABIN7538173)

Target: CD63

Protein-Typ: MNP Membrane Nanoparticle

Spezies: Human

Quelle: HEK-293 Cells

Produktdetails

Aufreinigungstag / Konjugat: Dieses CD63 Protein ist gelabelt mit DYKDDDDK Tag.

Verwendungszweck: Human CD63 full length protein membrane nanoparticles (MNPs)

Produktmerkmale: Plasma membrane-coated nanoparticles (MNPs) have been used in various applications, including delivery of therapeutic agents and induction of immune responses et al. Unlike the conventional strategies, MNPs directly leverage intact and natural functions of cell membranes, and show high biocompatibility, specificity, and low side effects. Our optimized MNPs platform for the full-length membrane protein production uses membrane coating technology and a HEK293 based expression platform. The high-purity plasma membrane-coated nanoparticles were produced by extrusion after membrane extraction from the host HEK293 cells containing the overexpressed target proteins.

Anwendungsinformationen

Kommentare:

Advantages of MNP:

• High display density of target membrane proteins
• Native structure and orientation of transmembrane protein
• soluble in aqueous solutions for routine biochemical analysis
• Detergent-free purification process
• Strong immunogenicity
• Works for MPs that can´t be produced via VLPs and EXos

Limitations of MNP:

• Lack of accurate quantification of the target membrane proteins.
• Need to develop special SPR assayx.
• Cell membranes contain housekeeping proteins that can result in immune response dilution.
• Some membrane proteins can´t be enriched on membrane.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please see Certificate of Analysis for specific instructions of reconstitution

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Haltbarkeit: 12 months

Antigendetails

Target: CD63

Andere Bezeichnung: CD63

Hintergrund: LAMP-3, ME491, MLA1, OMA81H, TSPAN30
The protein is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms.

Molekulargewicht: The human full length CD63 protein has a MW of 25.6 kDa

UniProt: P08962