RAB5 Protein (AA 1-215) (His tag)

Produktnummer ABIN7505252

Dieses Recombinant RAB5-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für RAB5 Protein (AA 1-215) (His tag) (ABIN7505252)

Target: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 1-215

Aufreinigungstag / Konjugat: Dieses RAB5 Protein ist gelabelt mit His tag.

Sequenz: Met 1-Asn 215

Produktmerkmale: A DNA sequence encoding the Human RAB5A protein (P20339) (Met 1-Asn 215) was expressed with a N-His&C-His tag.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Andere Bezeichnung: RAB5A

Hintergrund:

Abbreviation: RAB5A

Target Synonym: Ras-related protein Rab-5A,RAB5A

Background: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:16410077, PubMed:15378032). Contributes to the regulation of filopodia extension (PubMed:14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3.

Molekulargewicht:

Calculated MW: 23.54 kDa

Observed MW: 32 kDa

UniProt: P20339

Pathways: Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity