CD47 Protein (CD47) (AA 19-139)

Produktnummer ABIN7504274

Recombinant CD47-Protein exprimiert in HEK-293 Cells.

Kurzübersicht für CD47 Protein (CD47) (AA 19-139) (ABIN7504274)

Target: CD47

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Produktdetails

Proteineigenschaft: AA 19-139

Verwendungszweck: Human CD47 Protein

Sequenz: Gln19-Pro139

Produktmerkmale: Recombinant Human CD47 Protein is expressed from HEK293 without tag.It contains Gln19-Pro139.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CD47, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 0.24ug/ml determined by ELISA.,Human CD47 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human CD47 is greater than 95% as determined by SEC-HPLC.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: CD47

Andere Bezeichnung: CD47

Hintergrund: CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.

Molekulargewicht: 13.72 kDa. Due to glycosylation, the protein migrates to 35-48 kDa based on Bis-Tris PAGE result.

UniProt: Q08722