Nerve Growth Factor Protein (NGF)

Produktnummer ABIN7491868

Dieses Recombinant Nerve Growth Factor-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für Nerve Growth Factor Protein (NGF) (ABIN7491868)

Target: Nerve Growth Factor (NGF)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: Cell Culture (CC), Cytotoxicity Test (CyTox)

Reinheit: ≥98 % (HPLC)

Produktdetails

Verwendungszweck: Recombinant mouse proNGF protein

Sequenz: MEPYTDSNVP EGDSVPEAHW TKLQHSLDTA LRRARSAPTA PIAARVTGQT RNITVDPRLF KKRRLHSPRV LFSTQPPPTS SDTLDLDFQA HGTIPFNRTH RSKRSSTHPV FHMGEFSVCD SVSVWVGDKT TATDIKGKEV TVLAEVNINN SVFRQYFFET KCRASNPVES GCRGIDSKHW NSYCTTTHTF VKALTTDEKQ AAWRFIRIDT ACVCVLSRKA TRRG (the prodomain is shown in bold)

Spezifität: Toxin target: p75 neurotrophin receptor (p75NTR) and VPS10 domain-containing receptors (Sortilin and SorCS2)

Endotoxin-Niveau: <0.1 EU per 1 μg of the protein by the LAL method.

Biological Activity Comment: proNGF is the preferred ligand of p75NTR 1. In contrast to its mature form proNGF induces apoptosis via p75NTR 1, but can also mediate cell survival in DRGs2.

Anwendungsinformationen

Applikationshinweise: Antigen Preadsorption Control: 0.4 - 4 nM

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. Reconstitute in sterile, high-purity water at a concentration of at least 0.1 mg/mL. For long-term storage, aliquot the solution into small, single-use vials and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles to maintain optimal biological activity.

Buffer: Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).

Handhabung: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid multiple freeze-thaw cycles to maintain optimal biological activity,Avoid vigorous vortexing. protect from moisture

Lagerung: -20 °C

Informationen zur Lagerung: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C and protect from moisture.

Antigendetails

Target: Nerve Growth Factor (NGF)

Andere Bezeichnung: proNGF

Hintergrund: A recombinant mouse proNGF that binds TrkA, p75NTR, and SORT1 receptors involved in both cell survival and apoptosis

Molekulargewicht: 50.4 kDa (dimer)

Pathways: Regulation of Cell Size