SARS-CoV-2 Spike Protein (SARS-CoV-2 S) (AA 319-541, B.1.1.529 - Omicron, RBD) (Fc Tag)

Produktnummer ABIN7490663

Dieses Recombinant SARS-CoV-2 Spike-Protein wird in HEK-293 Cells exprimiert.

Kurzübersicht für SARS-CoV-2 Spike Protein (SARS-CoV-2 S) (AA 319-541, B.1.1.529 - Omicron, RBD) (Fc Tag) (ABIN7490663)

Target: SARS-CoV-2 Spike (SARS-CoV-2 S)

Protein-Typ: Recombinant

Spezies: SARS Coronavirus-2 (SARS-CoV-2)

Quelle: HEK-293 Cells

Applikation: Immunoassay (IA), Affinity Measurement (AM), Antibody Drug Assay (ADA), Screening Assay (ScA)

Reinheit: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Produktdetails

Proteineigenschaft: AA 319-541, B.1.1.529 - Omicron, RBD

Aufreinigungstag / Konjugat: Dieses SARS-CoV-2 Spike Protein ist gelabelt mit Fc Tag.

Verwendungszweck: Recombinant SARS-CoV-2 RBD(G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H) protein with C-terminal human Fc tag

Spezifität: S protein RBD(G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H)(Arg319-Phe541) hFc(Glu99-Ala330)

Produktmerkmale: Extracellular Domain Protein

Aufreinigung: Purified from cell culture supernatant by affinity chromatography

Anwendungsinformationen

Applikationshinweise: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Kommentare:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Haltbarkeit: 12 months

Antigendetails

Target: SARS-CoV-2 Spike (SARS-CoV-2 S)

Andere Bezeichnung: SARS-CoV-2 S protein

Substanzklasse: Viral Protein

Hintergrund: SARS-CoV-2 B.1.1.529 (Omicron) Spike RBD Protein
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as Covid19 (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. The spike protein is a type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which accounts for recognizing the cell surface receptor, ACE2. S2 contains basic elements needed for the membrane fusion. Recent publications indicate that S1-RBD domain can induce virus neutralizing-antibody and T cell response.

Molekulargewicht: predicted molecular mass of 51.2 kDa after removal of the signal peptide.The apparent molecular mass of RBD(G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H)-hFc is 55-70 kDa due to glycosylation.

UniProt: P0DTC2