Claudin 6 Protein-VLP (CLDN6) (AA 1-220)

Produktnummer ABIN7448159

Dieses VLP Claudin 6-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für Claudin 6 Protein-VLP (CLDN6) (AA 1-220) (ABIN7448159)

Target: Claudin 6 (CLDN6)

Protein-Typ: VLP

Biologische Aktivität: Active

Spezies: Cynomolgus

Quelle: HEK-293 Cells

Applikation: ELISA, Immunogen (Imm), Surface Plasmon Resonance (SPR), Functional Studies (Func)

Reinheit: > 95 % as determined by HPLC

Produktdetails

Proteineigenschaft: AA 1-220

Verwendungszweck: Cynomolgus Claudin 6 Protein-VLP

Sequenz: Met1 - Val220

Produktmerkmale: Recombinant Cynomolgus Claudin 6 Protein-VLP is expressed from HEK293.It contains Met1 - Val220.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus Claudin 6 VLP at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Claudin6 Antibody, mFc Tag with the EC50 of 4ng/ml determined by ELISA (QC Test). The affinity constant of 0.85 nM as determined in SPR assay (Biacore T200).

Anwendungsinformationen

Applikationshinweise:

• Antibody Discovery: Immunization, Screening, Functional Characterization
• Affinity determination: ELISA, SPR
• In vivo pharmacokinetic analysis
• CMC method development
• CAR-T Positive Rate Detection
• Blood sample determination: ELISA

Kommentare:

Virus-like particles (VLPs) are formed from the outer capsid protein of a virus and are tiny nanoparticles formed by the automatic assembly of one or more capsid proteins. VLPs do not contain viral infectious genomes, so they are relatively safe during production operations. The SAMS™ protein engineering platform has been used to express a series of biotinylated, non-biotinylated, and fluorescently-labeled VLP-displayed antigens. They are suitable for SPR, ELISA, CAR-T positive rate detection, and other experimental scenarios.

Virus-Like Particles (VLPs) are highly immunogenic, meaning that they can elicit a strong immune response in the host. VLPs are recognized by the immune system and are taken up by antigen-presenting cells (APCs) such as dendritic cells. Once taken up by APCs, VLPs are processed and presented to T cells, which can trigger the activation of B cells to produce antibodies against the displayed antigen. Because VLPs resemble the structure and composition of native viruses, they are highly effective at inducing both humoral and cellular immune responses.

Generally, VLPs range in size from approximately 20 to 200 nanometers (nm). Compared to a cell-based immunization approach, their smaller size can optimize the immune response to target the specific antigen displayed on the surface of the engineered VLPs.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Supplied as 0.22 μm filtered solution in PBS, 300 mM L-Arginine ( pH 7.4). Notice: If you need it for immunization, Do Not use any adjuvant.

Lagerung: -80 °C

Informationen zur Lagerung: Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Claudin 6 (CLDN6)

Andere Bezeichnung: Claudin 6

Hintergrund: Claudin-6 is a multipass transmembrane protein in the Claudin family. Claudin-6 is expressed by epithelial cells where it participates in tissue development and the maintenance of tight junction integrity. Human Claudin-6 shares 88 % and 86 % amino acid sequence identity with mouse and rat Claudin-6, respectively.

Molekulargewicht: 24.60 kDa.

UniProt: G7Q0B0

Pathways: Hepatitis C