TGFB3 Protein (AA 301-412)

Produktnummer ABIN7275712

Dieses Recombinant TGFB3-Protein wird in HEK-293 Cells exprimiert.

Kurzübersicht für TGFB3 Protein (AA 301-412) (ABIN7275712)

Target: TGFB3 (Transforming Growth Factor, beta 3 (TGFB3))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by Bis-Tris PAGE

Produktdetails

Proteineigenschaft: AA 301-412

Verwendungszweck: Human Mature TGF beta 3 Protein

Sequenz: Ala301-Ser412

Produktmerkmale: Recombinant Human Mature TGF beta 3 Protein is expressed from HEK293 without tag.It contains Ala301-Ser412.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human Mature TGF beta 3, No Tag at 1ug (100ul/Well) on the plate. Dose response curve for Human TGF-beta RII, hFc Tag with the EC50 of 10.1ng/ml determined by ELISA (QC Test).,Human Mature TGF beta 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured by its ability to inhibit the IL-4-dependent proliferation of TF-1 cells. The ED50 for this effect is 10-80 pg/ml (QC Test).,Loaded Human TGF-beta RII, hFc Tag on ProA-Biosensor can bind Human Mature TGF beta 3, No Tag with an affinity constant of 0.51 nM as determined in BLI assay (Gator® Prime).

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Dissolve the lyophilized protein in 4 mM HCL. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 4 mM HCl. Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: TGFB3 (Transforming Growth Factor, beta 3 (TGFB3))

Andere Bezeichnung: TGF beta 3

Hintergrund: Transforming growth factor-beta 3 is a cytokine which is involved in cell growth regulation and differentiation, stimulation of extracellular matrix and modulation of immune responses. TGF beta 3 RNA is expressed in a diverse array of tissues including perichondrium, bone, intervertebral discs, mesenteries, pleura, heart, lung, palate, and amnion, as well as in central nervous system (CNS) structures such as the meninges, choroid plexus and the olfactory bulbs. Furthermore, in several organ systems, TGF beta 3 transcripts are expressed during periods of active morphogenesis suggesting that the protein may be an important factor for the growth and differentiation of many embryonic tissues.

Molekulargewicht: 12.7 kDa. Due to glycosylation, the protein migrates to 13-15 kDa based on Bis-Tris PAGE result.

UniProt: P10600

Pathways: Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus