Latent TGF-beta 1 protein (His tag)

Produktnummer ABIN7275709

Produktdetails

Target: Latent TGF-beta 1

Protein-Typ: Recombinant

Spezies: Maus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: His tag

Sequenz: Leu30-Ser390

Reinheit: > 95% as determined by Tris-Bis PAGE

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Latent TGF-beta 1

Andere Bezeichnung: Latent TGF beta 1

Hintergrund: CEDLAP, DPD1, TGF beta1, TGFB, TGFB1, TGFbeta, TGF-beta-1,Latent TGF beta 1  cDNA encodes a 390 amino acid precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin-like convertase processes the proprotein to generate an N-terminal 249 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 1. Disulfide-linked homodimers of LAP and TGF- beta 1 remain non-covalently associated after secretion, forming the small latent TGF- beta 1 complex.

Molekulargewicht: 29.5/12.9 kDa. Due to glycosylation, the protein migrates to 40-50/14-15 kDa based on Tris-Bis PAGE result.

UniProt: P04202