Latent TGF-beta 1 (AA 30-390) protein (His-Avi Tag,Biotin)

Produktnummer ABIN7275708

Produktdetails

Target: Latent TGF-beta 1

Protein-Typ: Recombinant

Proteineigenschaft: AA 30-390

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: His-Avi Tag,Biotin

Verwendungszweck: Biotinylated Human Latent TGF beta 1/TGFB1 Protein

Sequenz: Leu30-Ser390 (C33S)

Produktmerkmale: Recombinant Biotinylated Human Latent TGF beta 1/TGFB1 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Leu30-Ser390(C33S).

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 93 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human ITGAV&ITGB6, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Tag with the EC50 of 65.7ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Latent TGF-beta 1

Andere Bezeichnung: Latent TGF beta 1

Hintergrund: Latent TGF beta 1 cDNA encodes a 390 amino acid precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin-like convertase processes the proprotein to generate an N-terminal 249 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 1. Disulfide-linked homodimers of LAP and TGF- beta 1 remain non-covalently associated after secretion, forming the small latent TGF- beta 1 complex.

Molekulargewicht: 44.2 kDa. Due to glycosylation, the protein migrates to 40-50 (LAP) /13-15 (Mature TGF beta 1) kDa based on Tris-Bis PAGE result.

UniProt: P01137