Latent TGF-beta 1 protein (His tag)
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- Target
- Latent TGF-beta 1
- Protein-Typ
- Recombinant
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Spezies
- Rhesusaffen
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Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- His tag
- Sequenz
- Leu30-Ser390(C33S)
- Reinheit
- > 95% as determined by Tris-Bis PAGE
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Rhesus macaque Latent TGF beta 1, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB6, His Tag with the EC50 of 1.2μg/ml determined by ELISA. See testing image for detail.
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Latent TGF-beta 1 (AA 30-390) protein (His tag)
Spezies: Human Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Latent TGF-beta 1 (AA 30-390) protein (His-Avi Tag,Biotin)Spezies: Human Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 93 % as determined by HPLC
Latent TGF-beta 1 (AA 30-390) (Active) protein (His tag)Spezies: Cynomolgus Wirt: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Latent TGF-beta 1 protein (His tag)Spezies: Maus Wirt: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
- Lagerung
- 4 °C,-80 °C
- Informationen zur Lagerung
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- Latent TGF-beta 1
- Andere Bezeichnung
- Latent TGF beta 1
- Hintergrund
- CEDLAP, DPD1, TGF beta1, TGFB, TGFB1, TGFbeta, TGF-beta-1,Latent TGF beta 1 cDNA encodes a 390 amino acid precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin-like convertase processes the proprotein to generate an N-terminal 249 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 1. Disulfide-linked homodimers of LAP and TGF- beta 1 remain non-covalently associated after secretion, forming the small latent TGF- beta 1 complex.
- Molekulargewicht
- 31.4/12.8 kDa. Due to glycosylation, the protein migrates to 40-50/14-15 kDa based on Tris-Bis PAGE result.
- UniProt
- F7HCV5
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