MMP 9 Protein (AA 20-730) (His tag)

Produktnummer ABIN7275274

Dieses Recombinant MMP 9-Protein wird in HEK-293 Cells exprimiert.

Kurzübersicht für MMP 9 Protein (AA 20-730) (His tag) (ABIN7275274)

Target: MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by Bis-Tris PAGE

Produktdetails

Proteineigenschaft: AA 20-730

Aufreinigungstag / Konjugat: Dieses MMP 9 Protein ist gelabelt mit His tag.

Verwendungszweck: Mouse MMP-9 Protein

Sequenz: Ala20-Pro730

Produktmerkmale: Recombinant Mouse MMP-9 Protein is expressed from HEK293 with His tag at the C-Terminus. The protein needs to be activated by APMA to have hydrolytic activity.It contains Ala20-Pro730.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse MMP-9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))

Andere Bezeichnung: MMP-9

Hintergrund: Matrix metalloproteinase 9 (MMP9) contributes to this process and deficiencies in the MMP9 lead to impaired healing. Inappropriate expression of MMP9 also contributes to impaired re-epithelialization. Previously we demonstrated that FOXO1 was activated in wound healing but to higher levels in diabetic wounds. To address mechanisms of impaired re-epithelialization we examined MMP9 expression in vivo in full thickness dermal scalp wounds created in experimental K14.

Molekulargewicht: 79.64 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result.

UniProt: P41245

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events