MMP8 Protein (AA 21-467) (His tag)

Produktnummer ABIN7275269

Recombinant MMP8-Protein exprimiert in HEK-293 Cells.

Kurzübersicht für MMP8 Protein (AA 21-467) (His tag) (ABIN7275269)

Target: MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Produktdetails

Proteineigenschaft: AA 21-467

Aufreinigungstag / Konjugat: Dieses MMP8 Protein ist gelabelt mit His tag.

Verwendungszweck: Human MMP-8 Protein (active form)

Sequenz: Phe21-Gly467

Produktmerkmale: Recombinant Human MMP-8 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe21-Gly467.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Anti-MMP-8 Antibody captured on CM5 Chip via Protein A can bind Human MMP-8, His Tag with an affinity constant of 1.14 uM as determined in SPR assay (Biacore T200).,Immobilized Human MMP-8, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MMP-8 Antibody, hFc Tag with the EC50 of 4.66ug/ml determined by ELISA (QC Test).,Human MMP-8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is > 300 pmoles/min/ug (QC Test).,The purity of Human MMP-8 is greater than 95% as determined by SEC-HPLC.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 50 mM Tris, 10 mM CaCl2, 150 mM NaCl ( pH 7.5). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))

Andere Bezeichnung: MMP-8

Hintergrund: Alteration of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) expression has been studied for various cardiac diseases, including dilated cardiomyopathy (DCM), with the significance of surrogate markers of extracellular matrix (ECM) remodeling. MMP-8 was identified only in myocardiocytes, while MMP-9 and TIMP-2 were present in both myocardiocytes and stroma, but with different intensity. The increasing intensity of MMP-8 and TIMP-2 immunoreactions was significantly associated with low HCS.

Molekulargewicht: 52.2 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Bis-Tris PAGE result.

UniProt: P22894