IgG1 Fc (AA 98-324) Protein
Kurzübersicht für IgG1 Fc (AA 98-324) Protein (ABIN7275250)
Target
Protein-Typ
Spezies
Quelle
Reinheit
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Proteineigenschaft
- AA 98-324
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Verwendungszweck
- Mouse IgG1 Fc Protein
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Sequenz
- Val98-Lys324
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Produktmerkmale
- Recombinant Mouse IgG1 Fc Protein is expressed from HEK293 without tag.It contains Val98-Lys324.
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Sterilität
- 0.22 μm filtered
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Endotoxin-Niveau
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- The affinity constant of 15.09 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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IgG1 Fc (AA 104-330) protein
Spezies: Human Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
IgG1 Fc protein (Fc Tag)Spezies: Maus Wirt: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE
IgG1 Fc proteinSpezies: Human Wirt: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. WB
IgG1 Fc (AA 100-330) protein (DYKDDDDK Tag)Spezies: Human Wirt: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Lagerung
- -20 °C,-80 °C
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Informationen zur Lagerung
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Haltbarkeit
- 12 months
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- IgG1 Fc
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Hintergrund
- Recombinant murine Fc fragment contains the hinge region, CH2, and CH3 region of the mouse IgG1 isotype. IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues.
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Molekulargewicht
- 25.7 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
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UniProt
- P01868
Target
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