IgG1 Fc (AA 98-324) Protein

Produktnummer ABIN7275250

Produktdetails

Target: IgG1 Fc

Protein-Typ: Recombinant

Proteineigenschaft: AA 98-324

Spezies: Maus

Quelle: HEK-293 Cells

Verwendungszweck: Mouse IgG1 Fc Protein

Sequenz: Val98-Lys324

Produktmerkmale: Recombinant Mouse IgG1 Fc Protein is expressed from HEK293 without tag.It contains Val98-Lys324.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: The affinity constant of 15.09 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: IgG1 Fc

Hintergrund: Recombinant murine Fc fragment contains the hinge region, CH2, and CH3 region of the mouse IgG1 isotype. IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues.

Molekulargewicht: 25.7 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.

UniProt: P01868