Klotho beta Protein (KLB) (His tag)
KLB
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
> 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
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- Target Alle Klotho beta (KLB) Proteine anzeigen
- Klotho beta (KLB)
- Protein-Typ
- Recombinant
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Spezies
- Human
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Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- Dieses Klotho beta Protein ist gelabelt mit His tag.
- Sequenz
- Met30-Thr983
- Reinheit
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human Beta Klotho at 2μg/ml (100μl/Well). Dose response curve for Anti-Beta Klotho Antibody1, hFc Tag with the EC50 of 38.5ng/ml determined by ELISA. The affinity constant of 0.54nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Klotho beta (KLB) (AA 53-994) protein (His tag)
KLB Spezies: Maus Wirt: HEK-293 Cells Recombinant > 90 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Lagerung
- 4 °C,-80 °C
- Informationen zur Lagerung
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- Klotho beta (KLB)
- Andere Bezeichnung
- Beta Klotho (KLB Produkte)
- Hintergrund
- BetaKlotho, beta-klotho, BKL, KLB, klotho beta like, Klotho beta, MGC142213,Beta-klotho (KLB) is a coreceptor required for endocrine fibroblast growth factor (FGF) 15/19 and FGF21 signaling in the brain. Klb is prominent within the hypothalamus, which is consistent with its metabolic functions, but diverse roles for Klb are now emerging. Central Klb expression is low but discrete and may govern FGF-targeted sites.
- Molekulargewicht
- 111.6 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result.
- UniProt
- Q86Z14
- Pathways
- Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Growth Factor Binding
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