Integrin alpha V beta 8 (ITGAV & ITGB8) Heterodimer (AA 31-992) protein (His-Avi Tag)
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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- Target
- Integrin alpha V beta 8 (ITGAV & ITGB8) Heterodimer
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 31-992
- Spezies
- Human
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Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- His-Avi Tag
- Verwendungszweck
- Human Integrin alpha V beta 8 (ITGAV & ITGB8) Heterodimer Protein
- Sequenz
- Phe31-Val992 (ITGAV) acidic tail & Glu43-Arg684 (ITGB8) basic tail
- Spezifität
- Uni-Prot: P06756-1 (ITGAV), P26012-1 (ITGB8)
- Produktmerkmale
- Recombinant Human Integrin alpha V beta 8 (ITGAV & ITGB8) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe31-Val992(ITGAV) acidic tail & Glu43-Arg684(ITGB8) basic tail.
- Reinheit
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human ITGAV&ITGB8, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Tag with the EC50 of 0.39ug/ml determined by ELISA. See testing image for detail.
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Integrin alpha V beta 8 (ITGAV & ITGB8) Heterodimer (AA 31-988) protein (His tag)
Spezies: Maus Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Haltbarkeit
- 12 months
-
- Target
- Integrin alpha V beta 8 (ITGAV & ITGB8) Heterodimer
- Hintergrund
- Deletions of the genes encoding the integrin αVβ8 (Itgav, Itgb8) have been shown to result in abnormal vascular development in the CNS, including prenatal and perinatal hemorrhage. Other work has indicated that a major function of this integrin in vivo is to promote TGFβ activation.
- Molekulargewicht
- 114 kDa (ITGAV)&76 kDa (ITGB8). Due to glycosylation, the protein migrates to 90-150 kDa based on Tris-Bis PAGE result.
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