IgG2a Fc (AA 98-330) (Active) Protein

Produktnummer ABIN7274869

Dieses Recombinant IgG2a Fc-Protein wird in HEK-293 Cells exprimiert.

Kurzübersicht für IgG2a Fc (AA 98-330) (Active) Protein (ABIN7274869)

Target: IgG2a Fc

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Produktdetails

Proteineigenschaft: AA 98-330

Verwendungszweck: Mouse IgG2a Fc Protein

Sequenz: Glu98-Lys330

Produktmerkmale: Recombinant Mouse IgG2a Fc Protein is expressed from HEK293 without tag.It contains Glu98-Lys330.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Mouse IgG2a Fc, No Tag with an affinity constant of 2.72 nM as determined in SPR assay (Biacore T200).,Mouse IgG2a Fc on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse IgG2a Fc is greater than 95% as determined by SEC-HPLC.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: IgG2a Fc

Hintergrund: Mouse IgG2a is one of the subtypes of murine immunoglobulin IgG which mediates immune responses to carbohydrate or polysaccharide antigens. The Fc region of IgG2a can bind to FcRn to prolong its half-life.

Molekulargewicht: 26.38 kDa. Due to glycosylation, the protein migrates to 33-38 kDa based on Bis-Tris PAGE result.

UniProt: P01863