IgG4 Fc (AA 99-326) (Active) Protein

Produktnummer ABIN7274868

Dieses Recombinant IgG4 Fc-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für IgG4 Fc (AA 99-326) (Active) Protein (ABIN7274868)

Target: IgG4 Fc

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Produktdetails

Proteineigenschaft: AA 99-326

Verwendungszweck: Human IgG4 Fc Protein

Sequenz: Glu99-Gly326

Produktmerkmale: Recombinant Human IgG4 Fc Protein is expressed from HEK293 without tag.It contains Glu99-Gly326.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IgG4 Fc, No Tag with an affinity constant of 1.365 uM as determined in SPR assay (Biacore T200).,Human IgG4 Fc on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human IgG4 Fc is greater than 95% as determined by SEC-HPLC.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: IgG4 Fc

Hintergrund: It is known as a IgG4-related disease and its differentiation is based on the analysis of IgG4 levels in the affected tissues. The IgG4-related disease is considered to be a generalized pathological process involving a wide spectrum of various disorders that may affect distant organs.

Molekulargewicht: 25.8 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Bis-Tris PAGE result.

UniProt: P01861