IgE Protein (AA 91-421) (His tag)

Produktnummer ABIN7274856

Dieses Recombinant IgE-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für IgE Protein (AA 91-421) (His tag) (ABIN7274856)

Target: IgE

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Produktdetails

Proteineigenschaft: AA 91-421

Aufreinigungstag / Konjugat: Dieses IgE Protein ist gelabelt mit His tag.

Verwendungszweck: Mouse IgE Protein

Sequenz: Val91-Ser421

Produktmerkmale: Recombinant Mouse IgE Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val91-Ser421.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1 EU per ug by the LAL method.

Biological Activity Comment: Immobilized Human Fc epsilon RI alpha, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse IgE, His Tag with the EC50 of 3.1ng/ml determined by ELISA.,Mouse IgE on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse IgE is greater than 95% as determined by SEC-HPLC.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: IgE

Hintergrund: Immunoglobulin E (IgE) is well known for its role in allergic disease, the manifestations of which are mediated through its two Fc receptors, FcεRI and CD23 (FcεRII). IgE and its interactions with these receptors are therefore potential targets for therapeutic intervention, and exciting progress has been made in this direction. Furthermore, recent structural studies of IgE-Fc, the two receptors, and of their complexes, have revealed a remarkable degree of plasticity at the IgE-CD23 interface and an even more remarkable degree of dynamic flexibility within the IgE molecule.

Molekulargewicht: 38.5 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.

UniProt: P06336