GDF15 Protein (AA 197-308) (Fc Tag,Biotin)

Produktnummer ABIN7274726

Recombinant GDF15-Protein exprimiert in HEK-293 Cells.

Kurzübersicht für GDF15 Protein (AA 197-308) (Fc Tag,Biotin) (ABIN7274726)

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Produktdetails

Proteineigenschaft: AA 197-308

Aufreinigungstag / Konjugat: Dieses GDF15 Protein ist gelabelt mit Fc Tag,Biotin.

Verwendungszweck: Biotinylated Human GDF15 Protein (Primary Amine Labeling)

Sequenz: Ala197-Ile308

Produktmerkmale: Recombinant Biotinylated Human GDF15 Protein (Primary Amine Labeling) is expressed from HEK293 with hFc tag at the N-Terminus.It contains Ala197-Ile308.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human GFRAL, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human GDF15, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Andere Bezeichnung: GDF15

Hintergrund: Growth and differentiation factor 15 (GDF15) is an inflammation-associated hormone with poorly defined biology. Here, we investigated the role of GDF15 in bacterial and viral infections. Inflammation induced GDF15, and that GDF15 was necessary for surviving both bacterial and viral infections, as well as sepsis. The protective effects of GDF15 were largely independent of pathogen control or the magnitude of inflammatory response, suggesting a role in disease tolerance.

Molekulargewicht: 37.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

UniProt: Q99988

Pathways: SARS-CoV-2 Protein Interaktom