FOLR1 Protein (AA 25-233) (His-Avi Tag)

Produktnummer ABIN7274667

Dieses Recombinant FOLR1-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für FOLR1 Protein (AA 25-233) (His-Avi Tag) (ABIN7274667)

Target: FOLR1 (Folate Receptor 1 (Adult) (FOLR1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by Tris-Bis PAGE, > 95 % as determined by HPLC

Produktdetails

Proteineigenschaft: AA 25-233

Aufreinigungstag / Konjugat: Dieses FOLR1 Protein ist gelabelt mit His-Avi Tag.

Verwendungszweck: Human FOLR1 Protein

Sequenz: Arg25-Met233

Produktmerkmale: Recombinant Human FOLR1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus., It contains Arg25-Met233.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human FOLR1, His Tag at 0.5μg/ml (100μl/Well) on plate.Dose response curve for Anti-FOLR1 Antibody, hFc Tag with the EC50 of 15ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: FOLR1 (Folate Receptor 1 (Adult) (FOLR1))

Andere Bezeichnung: FOLR1

Hintergrund: Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37 - 42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown. FOLR1 binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH .

Molekulargewicht: 27.5 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result.

UniProt: P15328

Pathways: Dicarboxylic Acid Transport