Fc epsilon RI/FCER1A Protein (AA 26-205) (His tag)

Produktnummer ABIN7274607

Produktdetails

Target: Fc epsilon RI/FCER1A (FCER1A) (Fc Fragment of IgE Receptor Ia (FCER1A))

Protein-Typ: Recombinant

Proteineigenschaft: AA 26-205

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses Fc epsilon RI/FCER1A Protein ist gelabelt mit His tag.

Verwendungszweck: Human Fc epsilon RI alpha/FCER1a Protein

Sequenz: Val26-Gln205

Produktmerkmale: Recombinant Human Fc epsilon RI alpha/FCER1a Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val26-Gln205.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human Fc epsilon RI alpha, His Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Fc epsilon RI alpha Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Fc epsilon RI/FCER1A (FCER1A) (Fc Fragment of IgE Receptor Ia (FCER1A))

Andere Bezeichnung: Fc epsilon RI alpha (FCER1A Produkte)

Hintergrund: Known susceptibility genes to atopy and asthma have been identified by linkage or associations with clinical phenotypes, including total serum IgE levels. IgE-mediated sensitivity reactions require a high-affinity IgE receptor (FcepsilonRI), which immobilizes the immunoglobulin on the surface of the effector cells, mostly mast cells and basophils. Similarly to the previously investigated beta subunit of the receptor, FCER1A is a good candidate for a quantitative trait locus (QTL) in allergic diseases, and appears to participate in the systemic regulation of IgE levels.

Molekulargewicht: 22.1 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on Tris-Bis PAGE result.

Pathways: Fc-epsilon Rezeptor Signalübertragung, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process