Endoglin Protein (ENG) (AA 26-586) (His-Avi Tag)

Produktnummer ABIN7274511

Dieses Recombinant Endoglin-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für Endoglin Protein (ENG) (AA 26-586) (His-Avi Tag) (ABIN7274511)

Target: Endoglin (ENG)

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Produktdetails

Proteineigenschaft: AA 26-586

Aufreinigungstag / Konjugat: Dieses Endoglin Protein ist gelabelt mit His-Avi Tag.

Verwendungszweck: Human Endoglin/CD105 Protein

Sequenz: Glu26-Gly586

Produktmerkmale: Recombinant Human Endoglin/CD105 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu26-Gly586.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human Endoglin, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Endoglin Antibody, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Endoglin (ENG)

Andere Bezeichnung: Endoglin

Hintergrund: Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins. Endoglin is a vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis. Required for normal structure and integrity of adult vasculature (PubMed:7894484). Regulates the migration of vascular endothelial cells. Required for normal extraembryonic angiogenesis and for embryonic heart development. May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis.

Molekulargewicht: 63.7 kDa. Due to glycosylation, the protein migrates to 70-82 kDa based on Tris-Bis PAGE result.

UniProt: P17813-1