SARS-CoV-2 Spike Protein (Trimer) (His-Avi Tag)

Produktnummer ABIN7274406

Dieses Recombinant SARS-CoV-2 Spike-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für SARS-CoV-2 Spike Protein (Trimer) (His-Avi Tag) (ABIN7274406)

Target: SARS-CoV-2 Spike

Protein-Typ: Recombinant

Spezies: SARS Coronavirus-2 (SARS-CoV-2)

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by Tris-Bis PAGE

Produktdetails

Proteineigenschaft: Trimer

Aufreinigungstag / Konjugat: Dieses SARS-CoV-2 Spike Protein ist gelabelt mit His-Avi Tag.

Verwendungszweck: SARS-COV-2 Spike S Trimer Protein

Sequenz: Val16-Glu1188

Produktmerkmale: Recombinant SARS-COV-2 Spike S is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Val16-Glu1188.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized SARS-COV-2 Spike S Trimer, His Tag at 1μg/ml (100μl/Well). Dose response curve for Human ACE2, hFc Tag with the EC50 of 42.2ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: SARS-CoV-2 Spike

Hintergrund: The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Molekulargewicht: 136.6 kDa. Due to glycosylation, the protein migrates to 175-230 kDa based on Tris-Bis PAGE result.