LI Cadherin Protein (AA 23-787) (His tag)

Produktnummer ABIN7274306

Recombinant LI Cadherin-Protein exprimiert in HEK-293 Cells.

Kurzübersicht für LI Cadherin Protein (AA 23-787) (His tag) (ABIN7274306)

Target: LI Cadherin

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by Bis-Tris PAGE

Produktdetails

Proteineigenschaft: AA 23-787

Aufreinigungstag / Konjugat: Dieses LI Cadherin Protein ist gelabelt mit His tag.

Verwendungszweck: Human CDH17/Cadherin 17 Protein

Sequenz: Gln23-Met787

Produktmerkmale: Recombinant Human CDH17/Cadherin 17 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gln23-Met787.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CDH17, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-CDH17 Antibody, hFc Tag with the EC50 of 4.1 ng/ml determined by ELISA.,Human CDH17 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Anti-CDH17 Ab., hFc Tag on ProA-Biosensor can bind Human CDH17, His Tag with an affinity constant of 2.26 nM as determined in BLI assay (Gator® Prime).

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: LI Cadherin

Andere Bezeichnung: CDH17

Hintergrund: Liver-intestine cadherin (CDH17) has been known to function as a tumor stimulator and diagnostic marker for almost two decades. In vivo studies showed CDH17 knockout resulted in apoptotic PC tumor death through activating caspase-3 activity. Taken together, CDH17 functions as an oncogenic molecule critical to PC growth by regulating tumor apoptosis signaling pathways and CDH17 could be targeted to develop an anti-PC therapeutic approach.

Molekulargewicht: 86.1 kDa. Due to glycosylation, the protein migrates to 90-115 kDa based on Bis-Tris PAGE result.

UniProt: Q12864