CD28H (AA 23-150) protein (His tag)

Produktnummer ABIN7274250

Dieses Recombinant -Protein wird in HEK-293 Cells produziert.

Kurzübersicht für CD28H (AA 23-150) protein (His tag) (ABIN7274250)

Target: CD28H

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Produktdetails

Proteineigenschaft: AA 23-150

Aufreinigungstag / Konjugat: His tag

Verwendungszweck: Human CD28H/IGPR-1 Protein

Sequenz: Leu23-Gly150

Produktmerkmale: Recombinant Human CD28H/IGPR-1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu23-Gly150.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per ug by the LAL method.

Biological Activity Comment: Immobilized Human CD28H, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human B7-H7, hFc Tag with the EC50 of 0.06μg/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: CD28H

Hintergrund: CD28H is constitutively expressed on all naive T cells. Repetitive antigenic exposure, however, induces a complete loss of CD28H on many T cells, and CD28H negative T cells have a phenotype of terminal differentiation and senescence. After extensive screening in a receptor array, a B7-like molecule, B7 homologue 5 (B7-H5), was identified as a specific ligand for CD28H.

Molekulargewicht: 15.1 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Tris-Bis PAGE result.

UniProt: Q96BF3-1