BCMA Protein (AA 1-54, Trimer) (Biotin,His-Avi Tag)

Produktnummer ABIN7273942

Dieses Recombinant BCMA-Protein wird in HEK-293 Cells exprimiert.

Kurzübersicht für BCMA Protein (AA 1-54, Trimer) (Biotin,His-Avi Tag) (ABIN7273942)

Target: BCMA (TNFRSF17) (Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by Bis-Tris PAGE

Produktdetails

Proteineigenschaft: AA 1-54, Trimer

Aufreinigungstag / Konjugat: Dieses BCMA Protein ist gelabelt mit Biotin,His-Avi Tag.

Verwendungszweck: Biotinylated Human BCMA/TNFRSF17 Trimer Protein

Sequenz: Met1-Ala54

Produktmerkmale: Recombinant Biotinylated Human BCMA/TNFRSF17 Trimer Protein expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met1-Ala54 trimer design.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human BAFF, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BCMA Trimer, His-Avi Tag with the EC50 of 11.1ng/ml determined by ELISA (QC Test).,Immobilized Anti-BCMA Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotunylated Human BCMA Trimer, His-Avi Tag with the EC50 of 1.9ng/ml determined by ELISA.,Biotunylated Human BCMA Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Human BAFF, monomeric hFc Tag on ProA-Biosensor can bind Biotinylated Human BCMA (Trimer), His-Avi Tag with an affinity constant of 0.15 nM as determined in BLI assay (Gator® Prime).

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS, 100 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: BCMA (TNFRSF17) (Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17))

Andere Bezeichnung: BCMA

Hintergrund: B-cell maturation antigen (BCMA or BCM), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17), is a protein that in humans is encoded by the TNFRSF17 gene.TNFRSF17 is a cell surface receptor of the TNF receptor superfamily which recognizes B-cell activating factor (BAFF).

Molekulargewicht: 29.5 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Bis-Tris PAGE result.

UniProt: Q02223