HLA-B*07:02 protein (SBP Tag)

Produktnummer ABIN7273184

Recombinant HLA-B*07:02-Protein exprimiert in Mammalian Cells.

Kurzübersicht für HLA-B*07:02 protein (SBP Tag) (ABIN7273184)

Target: HLA-B*07:02

Protein-Typ: Recombinant

Spezies: Human

Quelle: Mammalian Cells

Applikation: ELISA

Reinheit: > 80 %

Produktdetails

Aufreinigungstag / Konjugat: SBP Tag

Verwendungszweck: The product is HLA-B*07:02 complexed with β2-microglobulin (b2m)

Sequenz: heavy chain: GSHSMRYFYT SVSRPGRGEP RFISVGYVDD TQFVRFDSDA ASPREEPRAP WIEQEGPEYW DRNTQIYKAQ AQTDRESLRN LRGYYNQSEA GSHTLQSMYG CDVGPDGRLL RGHDQYAYDG KDYIALNEDL RSWTAADTAA QITQRKWEAA REAEQRRAYL EGECVEWLRR YLENGKDKLE RADPPKTHVT HHPISDHEAT LRCWALGFYP AEITLTWQRD GEDQTQDTEL VETRPAGDRT FQKWAAVVVP SGEEQRYTCH VQHEGLPKPL TLRWEPSSQS TVPI light chain: IQRTPKIQVY SRHPAENGKS NFLNCYVSGF HPSDIEVDLL KNGERIEKVE HSDLSFSKDW SFYLLYYTEF TPTEKDEYAC RVNHVTLSQP KIVKWDRDM Sequences without tags. Tag location is at the discretion of the manufactur er. If you have a special request, please contact us.

Spezifität: HLA-B*07:02 complexed with b2m, P01889 / P61769
HLA-B*07:02 presents peptides with the common motif of a proline residue at position 2 and a leucine residue at the c-terminal position 9, e.g. self-peptides derived from signal sequences or viral peptides derived from HIV or SARS-CoV-2.

Produktmerkmale: HLAs are produced in mammalian cell cultures as recombinant, truncated, soluble antigens, in complex with β2-microglobulin (HLA class I). As part of our quality control, all HLA are analyzed for purity by gel electrophoresis, concentration is determined by ELISA (0.5 mg/mL) and their functionality is verified with a specific antiserum.
contains endogenous peptides.

Aufreinigung: Chromatography

Anwendungsinformationen

Applikationshinweise: ELISA: 1 - 100 ng/mL

Kommentare:

reactive with antibodies W6,32,BBM.1,specific antiserum, B cell ELI Spot (predicted), in vitro memory B cell stimulation (predicted), HLA competition assay (predicted), control in various assays (predicted)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: TRIS based buffer, pH 8.0, Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shelf life: 6 month from date of production. Store at 2 °C - 8 °C

Haltbarkeit: 6 months

Antigendetails

Target: HLA-B*07:02

Hintergrund: Human Leukozyte Antigen, human Antigen-presenting major histocompatibility complex class I (MHCI) molecule. Expressed on all cells, displays peptides to CD8-positive T cells. Involved in cellular immune response.

Molekulargewicht: approx. 50 kDa (38 kDa heavy chain + 12 kDa b2m)

UniProt: P01889