BTK Kinase (Active) protein (His tag)

Produktnummer ABIN7317103

Dieses Recombinant BTK Kinase-Protein wird in Baculovirus infected Insect Cells produziert.

Kurzübersicht für BTK Kinase (Active) protein (His tag) (ABIN7317103)

Target: BTK Kinase

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Reinheit: > 85 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: His tag

Verwendungszweck: Recombinant Human Bruton Tyrosine Kinase/BTK Kinase Protein (His Tag)(Active)

Sequenz: Met 1-Ser 659

Produktmerkmale: A DNA sequence encoding the human BTK (NP_000052.1) (Met 1-Ser 659) was expressed with a C-terminal polyhistidine tag.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: 1. The specific activity was determined to be 115 nmol/min/mg using Poly(Glu,Tyr)4:1 peptide as substrate.2. Measured by its ability to bind human BLNK in a functional ELISA.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Frozen, Liquid

Buffer: Supplied as sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.0

Lagerung: -20 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Antigendetails

Target: BTK Kinase

Hintergrund:

Background: Bruton's tyrosine kinase (or BTK) is a type of kinase protein expressed in B lymphocytes and T cells. BTK contains a PH domain which binds phosphatidylinositol(3,4,5)-trisphosphate (PIP3). After binding to PIP3, BTK is induced to phosphorylate phospholipase C, which in turn hydrolyzes PIP2 into two second messagers, IP3 and DAG, which then modulate the activity of downstream proteins during B-cell signaling. Btk is also found implicated in the primary immunodeficiency disease X-linked agammaglobulinemia(Bruton's agammaglobulinemia). BTK played a key role in B-cell maturation as well as mast cell activation through the high-affinity IgE receptor. Patients with X-linked agammaglobulinemia have normal pre-B cell populations in their bone marrow but these B-cells can not mature and enter the circulation.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Synonym: AGMX1;AT;ATK;BPK;IMD1;PSCTK1;XLA

Molekulargewicht: 77.8 kDa

NCBI Accession: NP_000052