AMPK (A1/B2/G1) (Active) Protein

Produktnummer ABIN7317041

Recombinant AMPK (A1/B2/G1)-Protein exprimiert in Baculovirus infected Insect Cells.

Kurzübersicht für AMPK (A1/B2/G1) (Active) Protein (ABIN7317041)

Target: AMPK (A1/B2/G1)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Reinheit: > 94 % as determined by reducing SDS-PAGE.

Produktdetails

Verwendungszweck: Recombinant Human AMPK (G1/B2/A1) Heterotrimer Protein (Active)

Sequenz: Met 1-Pro 331

Produktmerkmale: A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1, A DNA sequence encoding the human PRKAB2 (O43741) (Met 1-Ile 272), constructed the plasmid 2, A DNA sequence encoding the human PRKAA1 (Q13131-1) (Met 1-Gln 559) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.

Anwendungsinformationen

Kommentare:

35, 37 & 95 kDa

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Frozen, Liquid

Buffer: Supplied as sterile 50 mM Tris, 200 mM NaCl, 2 mM GSH, 1 mM EDTA, 1 mM DTT, 10 % gly, pH 7.4

Lagerung: -20 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Antigendetails

Target: AMPK (A1/B2/G1)

Hintergrund: AMPK,AMPKa1

Molekulargewicht: 160 (38+30+92) kDa

UniProt: P54619