Tryptophan Synthetase protein (His tag)

Produktnummer ABIN7320985

Dieses Recombinant Tryptophan Synthetase-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für Tryptophan Synthetase protein (His tag) (ABIN7320985)

Target: Tryptophan Synthetase

Protein-Typ: Recombinant

Spezies: E. coli

Quelle: Escherichia coli (E. coli)

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: His tag

Verwendungszweck: Recombinant E.coli Tryptophan Synthase Protein (His Tag)

Sequenz: Met1-Ser268&Thr2-Ile397

Produktmerkmale: Recombinant E.coli Tryptophan synthase is produced by our E.coli expression system and the target gene encoding Met1-Ser268&Thr2-Ile397 is expressed with a 6His tag at the N-terminus.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Anwendungsinformationen

Kommentare:

28&40-50 kDa

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Lagerung: -20 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Antigendetails

Target: Tryptophan Synthetase

Andere Bezeichnung: Tryptophan Synthase

Hintergrund:

Background: Tryptophan synthase is a multienzyme α2β2 complex composed of two protein subunit. Tryptophan synthase catalyzes the last two steps in the synthesis of L-tryptophan (L-Trp). The α-subunit catalyzes cleavage of 3-indole-d-glycerol 3'-phosphate (IGP) to give indole and D-glyceraldehyde 3'-phosphate (G3P). Indole is then transferred through a 25-? hydrophobic tunnel to the β-subunit. The β2 subunit contains pyridoxal 5'-phosphate and catalyzes several pyridoxal 5'-phosphate-dependent reactions, including/3-elimination reactions 6 and a thiol-dependent transamination reaction. This enzyme is commonly found in Eubacteria, Archaebacteria, Protista, Fungi, and Plantae, but is absent from Animalia. As humans do not have tryptophan synthase, this enzyme has been explored as a potential drug target.

Synonym: Tryptophan synthetase, Tryptophan synthase

Molekulargewicht: 72.5 kDa