CD33L3 (Active) protein (Biotin)

Produktnummer ABIN7319803

Dieses Recombinant CD33L3-Protein wird in Human Cells exprimiert.

Kurzübersicht für CD33L3 (Active) protein (Biotin) (ABIN7319803)

Target: CD33L3

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Human Cells

Reinheit: >90 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Biotin

Verwendungszweck: Recombinant Human Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 (C-FC-Avi) Biotinylated

Sequenz: Phe20-Thr263

Produktmerkmale: Biotinylated Recombinant Human Sialic Acid-binding Ig-like Lectin 15 is produced by our Mammalian expression system and the target gene encoding Phe20-Thr263 is expressed with a Fc, Avi tag at the C-terminus.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Human Siglec-15-Fc-Avi (Cat#CY14) at 2μg/ml (100 μl/well) can bind Anti-Human Siglec15 mAb. The ED50 of Anti-Human Siglec15 mAb is 13.7 ng/ml.

Anwendungsinformationen

Kommentare:

58-75 kDa

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, 150 mM NaCl, 0.3 % Chaps, 5 % Trehalose, pH 7.4.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: CD33L3

Hintergrund:

Background: Human Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO--terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts.

Synonym: Sialic acid-binding Ig-like lectin 15, Siglec-15, CD33 antigen-like 3, CD33L3

Molekulargewicht: 55.3 kDa

UniProt: Q6ZMC9