Vinculin Protein (VCL) (His tag)
Kurzübersicht für Vinculin Protein (VCL) (His tag) (ABIN7318069)
Target
Alle Vinculin (VCL) Proteine anzeigenProtein-Typ
Spezies
Quelle
Reinheit
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Aufreinigungstag / Konjugat
- Dieses Vinculin Protein ist gelabelt mit His tag.
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Verwendungszweck
- Recombinant Human VCL/Vinculin Protein (His Tag)
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Sequenz
- Met 1-Gln 1066
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Produktmerkmale
- A DNA sequence encoding the human VCL (P18206-2) (Met 1-Gln 1066) was expressed, fused with a polyhistidine tag at the C-terminus.
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Sterilität
- 0.2 μm filtered
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Endotoxin-Niveau
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Vinculin (VCL) (AA 777-1066) protein (GST tag)
VCL Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Vinculin (VCL) (AA 2-235) protein (His-IF2DI Tag)VCL Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Vinculin (VCL) (AA 1-1066) protein (His tag)VCL Spezies: Maus Wirt: HEK-293 Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
Vinculin (VCL) (AA 1-1066) protein (GST tag)VCL Spezies: Human Wirt: Wheat germ Recombinant AP, AA, ELISA, WB
Vinculin (VCL) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)VCL Spezies: Human Wirt: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Buffer
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Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Haltbarkeit
- 12 months
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- Vinculin (VCL)
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Andere Bezeichnung
- VCL/Vinculin
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Hintergrund
- CMD1W,CMH15,HEL114,MV,MVCL,Metavinculin,VCL,Vinculin,Vinculin (VCL) is a cytoskeletal protein that is closely related to both cell-matrix interactions and cell-cell junctions. VCL is a membrane-cytoskeletal protein in focal adhesion plaques that is involved in linkage of integrin adhesion molecules to the actin cytoskeleton. The protein contains an acidic N-terminal domain and a basic C-terminal domain separated by a proline-rich middle segment. This protein has multi-ligand properties and has been found to interact with a number of microfilament associated proteins, such as talin, a-actinin, and paxillin, which reportedly bind to either the head or tail domains of vinculin.
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Molekulargewicht
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Calculated MW: 118 kDa
Observed MW: 115 kDa
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Gen-ID
- 7414
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UniProt
- P18206
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Pathways
- Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
Target
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