VEGF-165 / VEGF-A (AA 27-191) (Active) Protein

Produktnummer ABIN7319413

Dieses Recombinant VEGF-165 / VEGF-A-Protein wird in HEK-293 Cells exprimiert.

Kurzübersicht für VEGF-165 / VEGF-A (AA 27-191) (Active) Protein (ABIN7319413)

Target: VEGF-165 / VEGF-A

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Applikation: ELISA

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 27-191

Verwendungszweck: Recombinant Human VEGF165/VEGFA Protein

Sequenz: Ala27-Arg191

Produktmerkmale: Recombinant Human VEGF165 is produced by our Mammalian expression system and the target gene encoding Ala27-Arg191 is expressed.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 0.01 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Immobilized Human VEGF165 (PKSH033475) at 10μg/ml (100 μl/well) can bind Human VEGFR1-Fc (PKSH033445). The ED₅₀ of Recombinant Human VEGFR1-Fc (PKSH033445) is 36.4 ng/ml.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM Citrate, 8 % Sucrose, 4 % Mannitol, 0.05 % Tween 80, pH 4.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: VEGF-165 / VEGF-A

Andere Bezeichnung: VEGF165/VEGFA

Hintergrund: MVCD1,VEGF,VEGF165,VPF,Vascular Endothelial Growth Factor Isoform 165,Human Vascular endothelial growth factor (VEGF), also known as VEGF-A and vascular permeability factor (VPF), belongs to the platelet-derived growth factor family of cysteine-knot growth factors. It is a potent activator in vasculogenesis and angiogenesis both physiologically and pathologically. VEGF-A has 8 differently spliced isoforms, of which VEGF165 is the most abundant one. VEGF165 is a disulfide-linked homodimer consisting of two glycosylated 165 amino acid polypeptide chains. VEGF stimulates the cellular response through binding to tyrosine kinase receptors VEGFR1 and VEGFR2 on the cell surface. It is widely accepted that VEGFR2 mediate almost all of the known cellular responses to VEGF while the function of VEGFR1 is less defined and is thought to modulate the VEGFR2 signaling.

Molekulargewicht:

Calculated MW: 19.1 kDa

Observed MW: 18-24 kDa

Gen-ID: 7422

UniProt: P15692