TWF1 Protein (His-GST)

Produktnummer ABIN7317477

Dieses Recombinant TWF1-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für TWF1 Protein (His-GST) (ABIN7317477)

Target: TWF1 (Twinfilin, Actin-Binding Protein 1 (TWF1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: > 84 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses TWF1 Protein ist gelabelt mit His-GST.

Verwendungszweck: Recombinant Human TWF1/Twinfilin-1 Protein (His & GST Tag)

Sequenz: Met 1-Asp 252

Produktmerkmale: A DNA sequence encoding the human TWF1 isoform 4 (Q12792-4) (Met 1-Asp 252) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterilität: 0.2 μm filtered

Biological Activity Comment: Not validated for activity

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile 50 mM Tris, 500 mM NaCl, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: TWF1 (Twinfilin, Actin-Binding Protein 1 (TWF1))

Andere Bezeichnung: TWF1/Twinfilin-1

Hintergrund: A6,MGC23788,MGC41876,PTK9,Twinfilin-1, also known as Protein A6, Protein tyrosine kinase 9, TWF1 and PTK9, is a cytoplasm protein which belongs to theactin-binding proteins ADF family and Twinfilin subfamily. Twinfilin-1 (TWF1 / PTK9 ) is a highly conserved actin monomer-binding protein that regulates cytoskeletal dynamics in organisms from yeast to mammals. In addition to the mammalian twinfilin-1, a second protein with approximately 65 % sequence identity to twinfilin-1 exists in mouse and humans. TWF1 / PTK9 is expressed at high levels in the colon, testis, ovary, prostate and lung. It is expressed at lower levels in the brain, bladder and heart. It is not detected in liver. TWF1 / PTK9 is an actin-binding protein involved in motile and morphological processes. It inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. TWF1 / PTK9 seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles.

Molekulargewicht:

Calculated MW: 57.0 kDa

Observed MW: 50 kDa

Gen-ID: 5756

UniProt: Q12792

Pathways: Regulation of Actin Filament Polymerization, Maintenance of Protein Location