TIMP1 Protein

Produktnummer ABIN7317717

Dieses Recombinant TIMP1-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für TIMP1 Protein (ABIN7317717)

Target: TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 97 % as determined by reducing SDS-PAGE.

Produktdetails

Verwendungszweck: Recombinant Human TIMP-1/TIMP1 Protein (Active)

Sequenz: Cys 24-Ala 207

Produktmerkmale: A DNA sequence encoding the mature form of human TIMP1 (NP_003245.1) (Cys 24-Ala 207) was expressed.

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate MCA-PLGL-DPA-AR-NH2(R&D Systems, Catalog # ES001). The IC50 value is < 6 nM.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM NaAC, 200 mM NaCl, pH 5.5

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))

Andere Bezeichnung: TIMP-1/TIMP1

Hintergrund:

Background: TIMP metallopeptidase inhibitor 1, also known as TIMP-1/TIMP1, Collagenase inhibitor 16C8 fibroblast Erythroid-potentiating activity, TPA-S1TPA-induced proteinTissue inhibitor of metalloproteinases 1, is a natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. TIMP-1/TIMP1 is found in fetal and adult tissues. Highest levels are found in bone, lung, ovary and uterus. Complexes with metalloproteinases and irreversibly inactivates them by binding to their catalytic zinc cofactor. TIMP-1/TIMP1 mediates erythropoiesis in vitro, but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. In addition to its inhibitory role against most of the known MMPs, the protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this protein encoding gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This encoding gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. Complexes with metalloproteinases and irreversibly inactivates them by binding to their catalytic zinc cofactor. TIMP-1/TIMP1 is Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16.

Synonym: Metalloproteinase Inhibitor 1, Erythroid-Potentiating Activity, EPA, Fibroblast collagenase Inhibitor, Collagenase Inhibitor, Tissue Inhibitor of Metalloproteinases 1, TIMP-1, TIMP1, CLGI, TIMP,CLGI,EPA,EPO,HCI

Molekulargewicht: 21 kDa

NCBI Accession: NP_003245