s100a4 Protein (AA 2-101)

Produktnummer ABIN7197760

Dieses Recombinant s100a4-Protein wird in HEK-293 Cells exprimiert.

Kurzübersicht für s100a4 Protein (AA 2-101) (ABIN7197760)

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: HEK-293 Cells

Reinheit: > 85 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 2-101

Verwendungszweck: Recombinant Mouse S100A4 Protein

Sequenz: Ala2-Lys101

Produktmerkmale: A DNA sequence encoding the mouse S100A4 (P07091)(Ala2-Lys101) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Andere Bezeichnung: S100A4

Hintergrund: 18A2,42a,Capl,FSp1,Mts1,PeL98,metastasin,pk9a,S100A4, also known as metastasis-associated protein Mtsl, belongs to the family of small calcium-binding S100 proteins containing two EF-hand calcium-binding motifs. In humans at least 20 S100 family members that are distributed tissue specifically have been identified, and are involved in a number of cellular processes as transducers of calcium signal. S100A4 is a symmetric homodimer, and undergoes a relatively large conformational change upon the typical EF-hand binding calcium, which is necessary for S100A4 to interact with its protein targets and generate biological effects. It can bind the already known targets p53, F-actin, liprin β, myosin heavy chain II, and prevent their phosphorylation and multimerization. It has been demonstrated that S100A4 is directly involved in tumor metastasis including cell motility, invasion, apoptosis, angiogenesis and differentiation, and appears to be a metastasis factor and a molecular marker for clinical prognosis. Multiple alternatively spliced variants encoding the same protein have been identified.

Molekulargewicht:

Calculated MW: 11.7 kDa

Observed MW: 12 kDa

UniProt: P07091

Pathways: S100 Proteine