PTPN12 Protein (AA 1-355) (His-GST)

Produktnummer ABIN7317538

Dieses Recombinant PTPN12-Protein wird in Baculovirus infected Insect Cells produziert.

Kurzübersicht für PTPN12 Protein (AA 1-355) (His-GST) (ABIN7317538)

Target: PTPN12 (Protein Tyrosine Phosphatase G1 (PTPN12))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Reinheit: > 90 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 1-355

Aufreinigungstag / Konjugat: Dieses PTPN12 Protein ist gelabelt mit His-GST.

Verwendungszweck: Recombinant Human PTPN12 Protein (aa 1-355, His & GST Tag)

Sequenz: Met 1-Gln355

Produktmerkmale: A DNA sequence encoding the human PTPN12 (AAA36529.1) (Met1-Gln355) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to dephosphorylate a phosphotyrosine residue in an EGF receptor 988-998 phosphopeptide substrate, R&D Systems, Catalog # ES006. The specific activity is > 15 μmoles/min/mg.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: PTPN12 (Protein Tyrosine Phosphatase G1 (PTPN12))

Andere Bezeichnung: PTPN12

Hintergrund: PTP-PEST,PTPG1,PTPN12 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPN12 contains a C-terminal PEST motif, which serves as a protein-protein interaction domain, and may be related to protein intracellular half-life. PTPN12 was found to bind and dephosphorylate the product of oncogene c-ABL, thus may play a role in oncogenesis. PTPN12 was shown to interact with, and dephosphorylate, various of cytoskeleton and cell adhesion molecules, such as p130 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin, which suggested its regulatory roles in controlling cell shape and mobilit.

Molekulargewicht:

Calculated MW: 69.4 kDa

Observed MW: 64 kDa

Gen-ID: 5782

UniProt: Q05209