Podoplanin Protein (PDPN) (His-Fc tag)

Produktnummer ABIN7317674

Dieses Recombinant Podoplanin-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für Podoplanin Protein (PDPN) (His-Fc tag) (ABIN7317674)

Target: Podoplanin (PDPN)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Applikation: ELISA

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses Podoplanin Protein ist gelabelt mit His-Fc tag.

Verwendungszweck: Recombinant Human Podoplanin/PDPN Protein (His & Fc Tag)

Sequenz: Met 1-Lys 123

Produktmerkmale: A DNA sequence encoding the extracellular domain of human PDPN short isoform (Q86YL7-1) (Met 1-Lys 123) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Immobilized human Podoplanin at 10 μg/mL (100 μl/well) can bind biotinylated human CLEC1B-His, The EC₅₀ of biotinylated human CLEC1B-His is 0.4-1.0 μg/mL.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: Podoplanin (PDPN)

Andere Bezeichnung: Podoplanin/PDPN

Hintergrund: Aggrus,GP36,Glycoprotein 36,Gp36,PA2.26 Antigen,PDPN,Podoplanin,T1-Alpha,T1A,Podoplanin, also known asPDPN, is a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined. Alternatively spliced transcript variants encoding different isoforms have been identified.PDPN is a mucin-type glycoprotein negatively charged by extensive O-glycosylation and a high content of sialic acid, which expresses the adhesive property. It is selectively expressed in lymphatic endothelium as well as lymphangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. PDPN may contribute to form odontoblastic fiber or function as the anchorage to the tooth development and in proliferating epithelial cells of cervical loop and apical bud. The intensity of podoplanin expression is negatively correlated with the expression of CD34 and factor VIII. Podoplanin would be useful as a diagnostic marker for epithelioid hemangioendothelioma in liver tumors.

Molekulargewicht:

Calculated MW: 38.6 kDa

Observed MW: 50-55 kDa

Gen-ID: 10630

UniProt: Q86YL7

Pathways: Dicarboxylic Acid Transport