PDGF-AA Homodimer (Active) protein (His tag)

Produktnummer ABIN7318911

Dieses Recombinant -Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für PDGF-AA Homodimer (Active) protein (His tag) (ABIN7318911)

Target: PDGF-AA Homodimer

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: > 90 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: His tag

Verwendungszweck: Recombinant Human PDGF-AA Protein (His Tag)(Active)

Sequenz: Ser87-Thr 211

Produktmerkmale: Recombinant Human Platelet-derived growth factor AA is produced by our E.coli expression system and the target gene encoding Ser87-Thr211 is expressed with a 6His tag at the N-terminus.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured in a cell proliferation assay using BALB/C-3T3 mouse fibroblast cells.  The ED50 for this effect is 100-400 ng/ml.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: PDGF-AA Homodimer

Andere Bezeichnung: PDGF-AA

Hintergrund:

Background: Platelet-derived growth factor subunit A (PDGFA), belongs to the PDGF/VEGF growth factor family. PDGFA is a secreted protein, stored in platelet alpha-granules and released by platelets upon wounding. PDGFA is potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. It plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. PDGFA is required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis, normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. It plays an important role in wound healing, Signaling is modulated by the formation of heterodimers with PDGFB.

Synonym: Platelet-derived growth factor subunit A,PDGF subunit A,PDGF-1,Platelet-derived growth factor A chain,Platelet-derived growth factor alpha polypeptide, PDGFA,PDGF1

Molekulargewicht: 15.9 kDa

UniProt: P04085