PDE1B Protein (His-GST)

Produktnummer ABIN7317495

Dieses Recombinant PDE1B-Protein wird in Baculovirus infected Insect Cells produziert.

Kurzübersicht für PDE1B Protein (His-GST) (ABIN7317495)

Target: PDE1B (phosphodiesterase 1B, Calmodulin-Dependent (PDE1B))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Reinheit: > 94 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses PDE1B Protein ist gelabelt mit His-GST.

Verwendungszweck: Recombinant Human PDE1B Protein (His & GST Tag)

Sequenz: Met 1-Asp 536

Produktmerkmale: A DNA sequence encoding the human PDE1B long isoform (Q01064-1) (Met 1-Asp 536) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: PDE1B (phosphodiesterase 1B, Calmodulin-Dependent (PDE1B))

Andere Bezeichnung: PDE1B

Hintergrund: PDE1B1,PDES1B,Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B, also known as Cam-PDE 1B and PDE1B, is a cytoplasm protein which belongs to thecyclic nucleotide phosphodiesterase family and PDE1 subfamily. Phosphodiesterase-10A (PDE10A), Phosphodiesterase-1B (PDE1B), Phosphodiesterase-4B (PDE4B), and Phosphodiesterase-4A (PDE4A) are important regulators of signal transduction in striatum due to their catalysis of cyclic AMP and cyclic GMP. PDE1B is highly expressed in the striatum. It binds two divalent metal cations per subunit. Site one of PDE1B may preferentially bind zinc ions, while site two of PDE1B has a preference for magnesium and/or manganese ions. PDE1B is a cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. It has a preference for cGMP as a substrate.

Molekulargewicht:

Calculated MW: 89.2 kDa

Observed MW: 75 kDa

Gen-ID: 5153

UniProt: Q01064

Pathways: Neurotrophin Signalübertragung, cAMP Metabolic Process, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma