NOTCH2 Protein (His tag)

Produktnummer ABIN7319906

Produktdetails

Target: NOTCH2 (Notch 2 (NOTCH2))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Human Cells

Aufreinigungstag / Konjugat: Dieses NOTCH2 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human Notch2 (C-6His)

Sequenz: Leu26-Gln530

Produktmerkmale: Recombinant Human Neurogenic Locus Notch Homolog Protein 2 is produced by our Mammalian expression system and the target gene encoding Leu26-Gln530 is expressed with a 6His tag at the C-terminus.

Reinheit: >95 % as determined by reducing SDS-PAGE.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: NOTCH2 (Notch 2 (NOTCH2))

Andere Bezeichnung: Notch2 (NOTCH2 Produkte)

Hintergrund:

Background: Notch-2 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes. Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Human Notch-2 ECD (aa 26-530) shows 93 % , 93 %, 96 % and 96 % aa identity with the corresponding regions of mouse, rat, canine, and bovine Notch-2, respectively. Hajdu Cheney Syndrome (HCS) is a rare disease associated with mutations of NOTCH2 that lead to the translation of a truncated, presumably stable, NOTCH2 protein. NOTCH2 is down-regulated in colon cancer, and reduced expression is associated with a less differentiated, more aggressive phenotype, and reduced overall survival. NOTCH2 has also been shown to have pro-apoptotic and growth suppressive effects in thyroid carcinoma, and carcinoid tumors. NOTCH2 acts as an oncogene that promotes bladder cancer growth and metastasis through EMT, cell-cycle progression, and maintenance of stemness.

Synonym: AGS2, hN2, Notch homolog 2, Notch2, Notch-2, HJCYS

Molekulargewicht: 54.9 kDa

UniProt: Q04721

Pathways: Notch Signalweg, Stem Cell Maintenance