Neurturin Protein (NRTN) (AA 96-197) (His tag)

Produktnummer ABIN7318824

Dieses Recombinant Neurturin-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für Neurturin Protein (NRTN) (AA 96-197) (His tag) (ABIN7318824)

Target: Neurturin (NRTN)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Cell Culture (CC)

Reinheit: > 98 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 96-197

Aufreinigungstag / Konjugat: Dieses Neurturin Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human Neurturin/NRTN Protein

Sequenz: Ala96-Val197

Produktmerkmale: Recombinant Human Neurturin is produced by our E.coli expression system and the target gene encoding Ala96-Val197 is expressed.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 0.1 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measure by its ability to induce proliferation in SH-SY5Y cells. The ED₅₀ for this effect is <50 ng/mL.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: Neurturin (NRTN)

Andere Bezeichnung: Neurturin/NRTN

Hintergrund: NRTN,Neurturin,Neurturin is a member of the GDNF family of ligands, which include glial cell-derived neurotrophic factor (GDNF), Neurturin, Persephin, and Artemin. Neurturin is expressed in both neuronal and nonneuronal tissues. Similarly to other TGFβ family proteins, Neurturin is synthesized as a precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxyterminal domain. The carboxy terminal domain of Neurturin contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine-knot and the single interchain disulfide bond. Biologically active human Neurturin is a disulfide-linked homodimer of the carboxy-terminal 102 amino acid residues. Unlike other members of TGF-β family, bioactivities of all GDNF family ligands are mediated through a unique multicomponent receptor complex composed of high affinity ligand binding component (GFRα-1-GFRα-4) and a common signaling component (cRET receptor tyrosine kinase). Each member of the GDNF family ligands has its preferred binding protein. Neurturin preferentially binds to GFRα-2 but can also bind GFRα-1 at higher concentrations. It may play a role in regulating the development and maintenance of the central and peripheral nervous systems and as well as non neuronal systems.

Molekulargewicht:

Calculated MW: 12.6 kDa

Observed MW: 13 kDa

Gen-ID: 4902

UniProt: Q99748