TIMP2 Protein

Produktnummer ABIN7317884

Recombinant TIMP2-Protein exprimiert in HEK-293 Cells.

Kurzübersicht für TIMP2 Protein (ABIN7317884)

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 96 % as determined by reducing SDS-PAGE.

Produktdetails

Verwendungszweck: Recombinant Human TIMP2/TIMP-2 Protein (Active)

Sequenz: Cys 27-Pro 220

Produktmerkmale: A DNA sequence encoding the mature form of human TIMP2 (NP_003246.1) (Cys 27-Pro 220) was expressed and purified.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate MCA-PLGL-DPA-AR-NH2(R&D Systems, Catalog # ES001). The IC50 value is < 4 nM.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Andere Bezeichnung: TIMP2/TIMP-2

Hintergrund:

Background: Tissue inhibitors of metalloproteinases (TIMP) family are natural inhibitors of the matrix metalloproteinases (MMPs), the zinc enzymes involved in extracellular matrix maintenance and remodeling. The TIMP family encompasses four members (TIMP1-4), and they inhibit most MMPs by forming non-covalent binary complex. TIMP2 is a 22 kDa non N-glycosylated protein expressed by a variety of cell types, and plays a unique role among TIMP family members owing to its functions to regulate cellular responses to growth factors. Findings establish an unexpected, MMP-independent mechanism for TIMP2 inhibition of endothelial cell proliferation in vitro and reveal an important component of the antiangiogenic effect of TIMP2 in vivo. TIMP-2 thus is critical to the maintenance of tissue homeostasis and is involved in the regulation of tumor microenvironment.

Synonym: Metalloproteinase Inhibitor 2, CSC-21K, Tissue Inhibitor of Metalloproteinases 2,CSC-21K,DDC8

Molekulargewicht: 22 kDa

NCBI Accession: NP_003246

Pathways: cAMP Metabolic Process