KARS Protein (AA 2-597) (His tag)

Produktnummer ABIN7318750

Recombinant KARS-Protein exprimiert in HEK-293 Cells.

Kurzübersicht für KARS Protein (AA 2-597) (His tag) (ABIN7318750)

Target: KARS (Lysyl-tRNA Synthetase (KARS))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 2-597

Aufreinigungstag / Konjugat: Dieses KARS Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human KARS Protein (His Tag)

Sequenz: Ala2-Val597

Produktmerkmale: Recombinant Human Lysine--tRNA Ligase is produced by our Mammalian expression system and the target gene encoding Ala2-Val597 is expressed with a 6His tag at the C-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 20 % Glycerol, pH 8.0.

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: KARS (Lysyl-tRNA Synthetase (KARS))

Andere Bezeichnung: KARS

Hintergrund: KARS,KIAA0070,LysRS,Lysine--tRNA Ligase,Lysyl-tRNA Synthetase,Lysine-tRNA ligase, also known as Lysyl-tRNA synthetase, LysRS, KARS and KIAA0070, belongs to the class-II aminoacyl-tRNA synthetase family. The N-terminal cytoplasmic domain (1-65) is a functional tRNA-binding domain, which is required for nuclear localization, is involved in the interaction with DARS, but has a repulsive role in the binding to EEF1A1. A central domain (208-259) is involved in homodimerization and is required for interaction with HIV-1 GAG and incorporation into virions. KARS catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Defects in KARS are the cause of Charcot-Marie-Tooth disease recessive intermediate type B (CMTRIB).

Molekulargewicht:

Calculated MW: 69.1 kDa

Observed MW: 70-90 kDa

Gen-ID: 3735

UniProt: Q15046