Layilin Protein (LAYN) (AA 1-224)

Produktnummer ABIN7321083

Dieses Recombinant Layilin-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für Layilin Protein (LAYN) (AA 1-224) (ABIN7321083)

Target: Layilin (LAYN)

Protein-Typ: Recombinant

Spezies: Ratte

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 1-224

Verwendungszweck: Recombinant Rat Layilin/LAYN Protein

Sequenz: Met1-Glu224

Produktmerkmale: A DNA sequence encoding the rat LAYN (NP_001178926.1) (Met1-Glu224) was expressed with six amino acids (LEVLFQ) at the C-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: Layilin (LAYN)

Andere Bezeichnung: Layilin/LAYN

Hintergrund: LAYN,Layilin, a recently characterized as a 55 kDa transmembrane protein with homology to C-type lectins, is present in numerous cell lines and tissue extracts. As one of the adaptor proteins, talin mediates the interactions between the actin filaments and the cell membrane by binding to integral membrane proteins and to the cytoskeleton. Layilin is a newly identified membrane-binding site for talin in peripheral ruffles of spreading cells, a ten-amino acid motif in the layilin cytoplasmic domain is sufficient for talin binding, and its adjacent LH2-LH3 tandem arrays in the cytoplasmic domain provide docking sites for talin. Layilin binds specifically to hyaluronan (HA) through its extracellular domain, a ubiquitous extracellular matrix component in most animal tissues and body fluids, but not to other tested glycosaminoglycans. The research suggests that layilin may mediate signals from extracellular matrix to the cell cytoskeleton via interaction with different intracellular binding partners and thereby be involved in the modulation of cortical structures in the cell. All the above actions reveal an interesting parallel between layilin and the known HA receptor CD44. In addition, merlin and radixin have been identified as different intracellular binding partners of layilin. Accordingly, it has been suggested that layilin plays roles in a variety of cellular processes, including cell shape, adhesion, motility, and homeostasis, as well as signal transduction. In addition, layilin might play an important role in the process of invasion and lymphatic metastasis of lung carcinoma.

Molekulargewicht:

Calculated MW: 23.7 kDa

Observed MW: 34 kDa

NCBI Accession: NP_001178926